I’m pleased to announced that two pieces of work I’ve co-authored are now live on bioRxiv:

In the first, “Two subsets of human marginal zone B cells resolved by global analysis of lymphoid tissues and blood“, from Jacqueline Siu, we look at human B cell differentiation and dissemination using multiple different technologies, and uncover two distinct subsets of marginal zone B cells. We explore the differences between these subsets in terms of cell surface and transcriptomic profiles, clonal dissemination, hypermutation frequency, and micro-anatomical distribution in healthy spleen, mesenteric lymph node and appendix, and compare them in the blood of healthy patients and those with lupus.

In the second, “A SIMPLI (Single-cell Identification from MultiPLexed Images) approach for spatially resolved tissue phenotyping at single-cell resolution”, from the Ciccarelli Lab, presents a novel technology-agnostic software that unifies all steps of multiplexed imaging data analysis. This new software is shown in action by studying the human colon mucosa for IgA and the human appendix for T follicular helper cells.